Prodchist ma'lumot
Mahsulot haqida ma'lumot
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Oqsil to'liq ismi |
Catenin beta -1} |
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Sinonimlar |
Beta Catenin, beta-Catenin, CTNNB, CTNNB1, CTNNB1, CTNNNB, - CTHHARIN, Cacerillo, Catenin Beta 1, Catnb |
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Immunenen |
KLH sichqonchaning beta-kateninga mos keladigan konjugalli peptid |
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Unprot identifikatori |
Q02248 |
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Izotip |
Xo'rlamoq |
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Poklik |
Yaqinliklarni tozalash |
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BUMIYALAR |
Sitoplazma, yadro, hujayralar |
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Bashorat qilingan MV. \/ Kuzatilgan mw. |
85 KDA \/ 92 KDA |
Arizalar
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Jufton |
Odam, sichqoncha, kalamush |
1: 500-1: 1000 |
miya |
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IHC \/ agar bo'lsa |
Odam, sichqoncha, kalamush |
1: 500-1: 2000 |
yo'g'on ichak, oshqozon |
Fon
-Catenin - bu hujayra hujayralari va gen transkori transkripsiyasini tartibga solish va muvofiqlashtirish bilan shug'ullanadigan ikki tomonlama oqsil. Odamlarda CTNNB1 oqsil CTNNB1 geni tomonidan kodlanadi. Druropilada gomomol oqsil deyiladi. -Catenin - bu Kadherin oqsillari majmuasining pastki qismidir Bu "Catenin" oqsillari oilasi va "Tcakenin" ga, shuningdek, Plakoglobin deb nomlanuvchi "Kankenin" ga ega.
Rasmlar
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WB -CENIN-kodni tahlil qilish (GB11015). Namuna: RIPA Lisis Buferi (G2002) tomonidan davolash oqsillangan. Bloklash buferi: 3% bo'lmagan quruq sut (GC310001) TBST, RT, 1 soat. Birlamchi antikod: 1: 1000, 4 daraja. Ikkilamchi antikod: HRP echki Anti-Rabbit LGG (Gb23303), 1: 3000, RT, 1 soat.
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IHC tahlili (GB11015). Namuna: inson oshqozon (kerosin), 4% PFA (G1101) 12-24 H. Antijenni qidirish: sitrat buferi (ph 6. 0}) (G1 {1}} 0), 98 daraja, 20 minut. Bloklash buferi: 3% BSA (GC305010), RT, 30 minut. Birlamchi antikor: 1: 1500, 4 daraja. Ikkilamchi antikod: HRP echki Anti-Rabbit LGG (Gb23303), 1: 200 RT 1H.
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Agar -Cateninni tahlil qilish (GB11015). Namuna: Inson yo'g'onligi (kerosin), 4% PFA (G1101) 12-24 H. Antijen Yangilik: Te buferi (PH 9. {1}}) (G1203), 98 daraja, 20min. Bloklash buferi: 3% BSA (GC305010), RT, 30 minut. Birlamchi antikody: 1: 500, 4 daraja. Ikkilamchi antikoba: cy3 concubate Accesson Anticuit Ig (H + L3) (Gb21303), 1: 300 RT, 1 soat. |
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Agar -Cateninni tahlil qilish (GB11015). Namuna: inson oshqozon (kerosin), 4% PFA (G1101) 12-24 H. Antijen Yangilik: Te buferi (PH 9. {1}}) (G1203), 98 daraja, 20min. Bloklash buferi: 3% BSA (GC305010), RT, 30 minut. Birlamchi antikody: 1: 500, 4 daraja. Ikkilamchi antikoba: cy3 concubate Accesson Anticuit Ig (H + L3) (Gb21303), 1: 300 RT, 1 soat. |
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Agar -Cateninni tahlil qilish (GB11015). Namuna: Sichqoncha (kerosin), 4% PFA (G1101) 12-24 H. Antijen Yangilik: Te buferi (PH 9. {1}}) (G1203), 98 daraja, 20min. Bloklash buferi: 3% BSA (GC305010), RT, 30 minut. Birlamchi antikod: 1: 200, 4 daraja. Ikkilamchi antikoba: cy3 concubate Accesson Anticuit Ig (H + L3) (Gb21303), 1: 300 RT, 1 soat. |
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Agar -Cateninni tahlil qilish (GB11015). Namuna: kalamushning oshqozon (kerosin), 4% PFA (G1101) 12-24 H. Antijen Yangilik: Te buferi (PH 9. {1}}) (G1203), 98 daraja, 20min. Bloklash buferi: 3% BSA (GC305010), RT, 30 minut. Birlamchi antikod: 1: 200, 4 daraja. Ikkilamchi antikoba: cy3 concubate Accesson Anticuit Ig (H + L3) (Gb21303), 1: 300 RT, 1 soat.
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Saqlash
| Saqlash | -20 darajasida bir yil. Takroriy muzlatgich \/ aylanishini tsikllardan saqlaning. |
| Saqlash buferi | 0 bilan. 02% natriy azid, 100 UG \/ ml BSA va 50% glitserin. |
ESLATMA:
1.Bu mahsulot faqat tadqiqot uchun mo'ljallangan.
2.Bu mahsulotni birlamchi antibodli antibod buferi (G2025) bilan suyultirish tavsiya etiladi.
Issiq teglar: Beta-beta-beta-Betin quyon pab, Xitoy beta-beta-Betin quyon pab ishlab chiqaruvchilari, etkazib beruvchilar, zavod
